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Journal / Immunology and Infection / En real-time styrke assay for Chimeric antigen…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
En real-time styrke assay for Chimeric antigen receptor T celler rettet mod solide og hæmatologiske cancer celler
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En real-time styrke assay for Chimeric antigen receptor T celler rettet mod solide og hæmatologiske cancer celler

Article DOI: 10.3791/59033-v • 08:46 min • November 12th, 2019
November 12th, 2019 •
1, 2, 2, 2, 2, 2, 2, 2, 2, 1,3, 1, 1, 1, 2
1ACEA Biosciences, Inc, 2ProMab Biotechnologies. Inc, 3ACEA Therapeutics

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Vi beskriver en kvantitativ Real-Time in vitro cytolyse assay system til at evaluere styrken af chimerisk antigen receptor T celler rettet mod flydende og solide tumorceller. Denne protokol kan udvides til at vurdere andre immun Effector celler, samt Kombinationsbehandlinger.

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Immunologi og infektion kimerisk antigen receptor adoptiv celleterapi lentiviral genoverførsel T-celler kræft immunterapi rtca in vitro funktionel analyse cytotoksicitet styrke
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