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Journal / Biochemistry / Identifikation af roman CK2 Kinase substrater ved …
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Biochemistry

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JoVE Journal Biochemistry
Identifikation af roman CK2 Kinase substrater ved hjælp af en alsidig biokemiske tilgang
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Identifikation af roman CK2 Kinase substrater ved hjælp af en alsidig biokemiske tilgang

Article DOI: 10.3791/59037-v • 11:11 min • February 21st, 2019
February 21st, 2019 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

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Formålet med denne protokol er at mærke, berige og identificere substrater af protein kinase CK2 fra en kompleks biologisk prøve celle lysate f.eks væv homogenatet. Denne metode udnytter unikke aspekter af CK2 biologi til dette formål.

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Biokemi spørgsmålet 144 kasein kinase 2 CK2 protein kinase substrat identifikation fosforylering kinase assay kemisk biologi
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