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Journal / Biochemistry / Bruger i Vitro fluorescens resonans…
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Biochemistry

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JoVE Journal Biochemistry
Bruger i Vitro fluorescens resonans energioverførsel at studere dynamikken i proteinkomplekser på en millisekund tidsskala
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Journal (Biochemistry)
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Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
 
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Bruger i Vitro fluorescens resonans energioverførsel at studere dynamikken i proteinkomplekser på en millisekund tidsskala

Article DOI: 10.3791/59038-v • 10:50 min • March 14th, 2019
March 14th, 2019 •
1,3, 2,3, 1,3
1Department of Biochemistry, Purdue University, 2Department of Botany and Plant Pathology, Purdue University, 3Center for Plant Biology, Purdue University

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Protein-protein interaktioner er kritisk for biologiske systemer, og undersøgelser af bindende kinetik give indsigt i den dynamik og funktion af proteinkomplekser. Vi beskriver en metode, der kvantificerer kinetiske parametre for et proteinkompleks ved hjælp af fluorescens resonans energioverførsel og stoppede strømmen teknik.

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Biokemi sag 145 protein kompleks bindende kinetik protein mærkning in vitro-ÆRGRE stoppede strømmen fluorescens protein exchange
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