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Journal / Biochemistry / Bruke i Vitro fluorescens resonans…
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Biochemistry

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JoVE Journal Biochemistry
Bruke i Vitro fluorescens resonans energioverføring å studere dynamikken i Protein komplekser på et millisekund tidsskala
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Bruke i Vitro fluorescens resonans energioverføring å studere dynamikken i Protein komplekser på et millisekund tidsskala

Article DOI: 10.3791/59038-v • 10:50 min • March 14th, 2019
March 14th, 2019 •
1,3, 2,3, 1,3
1Department of Biochemistry, Purdue University, 2Department of Botany and Plant Pathology, Purdue University, 3Center for Plant Biology, Purdue University

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Protein-protein interaksjoner er avgjørende for biologiske systemer, og studier av bindende kinetics gir innsikt i dynamikk og funksjonen av protein komplekser. Vi beskriver en metode som quantifies kinetic parameterne for et protein kompleks fluorescens resonans energioverføring og stoppet-flow teknikken.

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Biokjemi problemet 145 protein kompleks bindende kinetikk protein merking i vitro bånd stoppet-flow fluorescens protein exchange
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