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Journal / Bioengineering / Behandeling en evaluatie van menselijke primaire…
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Bioengineering

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JoVE Journal Bioengineering
Behandeling en evaluatie van menselijke primaire prostaat Organoid cultuur
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Behandeling en evaluatie van menselijke primaire prostaat Organoid cultuur

Article DOI: 10.3791/59051-v • 08:45 min • January 17th, 2019
January 17th, 2019 •
*1, *1, 1, 1,2,3, 1,3
1Department of Pathology, University of Illinois at Chicago, 2Departments of Urology, Physiology, and Biophysics, University of Illinois at Chicago, 3University of Illinois Cancer Center
* These authors contributed equally

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Hier presenteren we een protocol om begeleiden van menselijke primaire prostaat organoid behandeling dan eindpunten om te beoordelen fenotype suggereren. Zaaien, cultuur onderhoud, herstel van matrix gel, worden morfologische kwantificering, insluiten en segmenteren, FFPE segmenteren, geheel-mount kleuring en toepassing van commerciële tests beschreven.

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Bioengineering kwestie 143 Organoid eindpunt 3D quanification beeldanalyse FFPE geheel-mount morfologie prostaat mens immunofluorescentie
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