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Syntese og Bioconjugation Thiol-reaktive reagenser for etableringen av nettstedet-selektivt endret Immunoconjugates
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Syntese og Bioconjugation Thiol-reaktive reagenser for etableringen av nettstedet-selektivt endret Immunoconjugates

Article DOI: 10.3791/59063-v • 08:47 min • March 6th, 2019
March 6th, 2019 •
1, 1,2, 1, 1,2,3,4
1Department of Chemistry, Hunter College of the City University of New York, 2Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 3Department of Radiology, Memorial Sloan Kettering Cancer Center, 4Department of Radiology, Weill Cornell Medical College

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I denne protokollen, vil vi beskrive syntesen av PODS, en phenyoxadiazolyl methyl sulfone-baserte reagens for området-selektiv feste Last til thiols av biomolecules, spesielt antistoffer. I tillegg vil vi beskrive syntese og karakterisering av en PODS-bærende bifunctional chelator og dens Bøyning til en modell antistoff.

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Kjemi problemet 145 områdespesifikk bioconjugation området-selektive bioconjugation maleimide thiol sulfhydryl radioimmunoconjugate immunoconjugate
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