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Journal / Genetics / Karaktärisera Histon posttranslationella…
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Genetics

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JoVE Journal Genetics
Karaktärisera Histon posttranslationella modifieringen förändringar i jäst neurodegenerativa Proteinopathy modeller
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Karaktärisera Histon posttranslationella modifieringen förändringar i jäst neurodegenerativa Proteinopathy modeller

Article DOI: 10.3791/59104-v • 08:33 min • March 24th, 2019
March 24th, 2019 •
1,2, 1,3, 1, 1, 1, 1, 1,4
1Chemistry Department, Brooklyn College, 2Ph.D. Program in Biochemistry, Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology, Graduate Center of the City University of New York

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Detta protokoll beskriver experimentella procedurer för att karakterisera genome-wide förändringar i nivåerna av Histon post-translationella modifieringar (PTM) som förekommer i samband med överuttryck av proteiner associerade med ALS och Parkinsons sjukdom i Saccharomyces cerevisiae modeller. Efter SDS-PAGE separation upptäcks enskilda Histon PTM halter med modifiering-specifika antikroppar via Western blotting.

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Genetik fråga 145 neurodegeneration amyotrofisk lateral skleros Parkinsons sjukdom α-synuclein smält i sarkom tjära DNA-bindande protein 43 Histon post-translationella modifieringar epigenetik
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