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JoVE Journal Biochemistry
Analys av Endocytic upptag och retrograd Transport till Trans-Golgi nätverket med Functionalized Nanobodies i odlade celler
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Analys av Endocytic upptag och retrograd Transport till Trans-Golgi nätverket med Functionalized Nanobodies i odlade celler

Article DOI: 10.3791/59111-v • 11:05 min • February 21st, 2019
February 21st, 2019 •
1, 1
1Biozentrum, University of Basel

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Retrograd transporten av proteiner från cellytan till Golgi är viktigt att upprätthålla membran homeostas. Här, beskriver vi en metod för att analysera biokemiskt cell surface-till-Golgi transport av rekombinanta proteiner med functionalized nanobodies i HeLa cells.

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Immunologi och infektion fråga 144 nanobodies retrograd transport Golgi komplexet tyrosin sulfatering bakteriell uttryck radiolabeling andra HeLa celler
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