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Journal / Developmental Biology / Fare Kraniyofacial dokularda ve Undecalcified…
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Developmental Biology

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JoVE Journal Developmental Biology
Fare Kraniyofacial dokularda ve Undecalcified kemik doku hazırlama ve Immünostasyon
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Fare Kraniyofacial dokularda ve Undecalcified kemik doku hazırlama ve Immünostasyon

Article DOI: 10.3791/59113-v • 10:03 min • May 10th, 2019
May 10th, 2019 •
*1,2, *2, 2
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine of Ministry of Education, School and Hospital of Stomatology, Wuhan University, 2Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan
* These authors contributed equally

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Burada, kraniyofasiyal morphogenesis/patogenezi sırasında protein düzeylerini algılamak ve ölçmek için ayrıntılı bir protokol sunuyoruz Buna ek olarak, immünostasyon için genç farelerden undecalcified Sert dokularda hazırlama ve donuk kesit için bir yöntem açıklanmaktadır.

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Gelişim biyolojisi sayı 147 gelişimsel biyoloji transgenik fareler BMP sinyalizasyon immünostenleme kraniyofacial morfojenik undecalcified sert dokular miktar
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