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Agrobacterium tumefaciens e Agrobacterium rhizogenes-transformação mediada de batata e a atividade de promotor de um Gene de suberina por GUS Staining
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Agrobacterium tumefaciens e Agrobacterium rhizogenes-transformação mediada de batata e a atividade de promotor de um Gene de suberina por GUS Staining

Article DOI: 10.3791/59119-v • 08:31 min • March 29th, 2019
March 29th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Biology, Universitat de Girona

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Aqui, apresentamos dois protocolos para transformar plantas de batata. A transformação de Agrobacterium tumefaciens leva a uma planta transgénica completa enquanto o Agrobacterium rhizogenes produz raízes peludas transgênicas em uma selvagem tipo de fotos que podem ser propagados Self. Nós então detectar atividade de promotor por GUS coloração nas raízes transformadas.

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Ciências do ambiente edição 145 Agrobacterium rhizogenes Agrobacterium tumefaciens Solanum tuberosum suberina raiz batata
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