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Journal / Genetics / ATAC-seq Assay med lav mitokondriel DNA…
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JoVE Journal Genetics
ATAC-seq Assay med lav mitokondriel DNA forurening fra primære menneskelige CD4 + T-lymfocytter
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ATAC-seq Assay med lav mitokondriel DNA forurening fra primære menneskelige CD4 + T-lymfocytter

Article DOI: 10.3791/59120-v • 08:36 min • March 22nd, 2019
March 22nd, 2019 •
1, 1,2, 1,3
1Department of Biology, Boston University, 2Department of Computer Science and Engineering, University of California San Diego, 3Bioinformatics Program, Boston University

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Vi præsenterer her, en protokol for at udføre en analyse for transposase-tilgængelige kromatin sekventering (ATAC-seq) på aktiverede CD4 + humane lymfocytter. Protokollen er blevet ændret for at minimere kontaminering af mitokondriel DNA læsninger fra 50% til 3% gennem indførelse af en ny lysisbuffer.

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Genetik sag 145 Epigenomics ATAC-FF. Tn5 CD4 + lymfocytter kerner mitokondriel DNA forurening
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