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Journal / Immunology and Infection / Einzelne Kette MHC-Technologie zur Co-Agonismus…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Einzelne Kette MHC-Technologie zur Co-Agonismus in menschliche CD8 + T-Zell-Aktivierung zu untersuchen
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Einzelne Kette MHC-Technologie zur Co-Agonismus in menschliche CD8 + T-Zell-Aktivierung zu untersuchen

Article DOI: 10.3791/59126-v • 12:09 min • February 28th, 2019
February 28th, 2019 •
1, 1, 1, 1,2, 3, 4, 3, 1,2,5, 6, 1, 1,2,5
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, 4Singapore Immunology Network, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, 6Department of Immunology, Wright-Fleming Institute, Imperial College London

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Dieses Protokoll beschreibt die Verwendung der einzelnen Kette MHC Klasse I komplexe molekulare Interaktionen in menschliche CD8 + T-Zell-Aktivierung zu untersuchen: Generation von veränderter antigenpräsentierende Zellen mit dem Ausdruck einzelne Kette konstruiert, Kultur der menschlichen CD8 + T Zelle Klon und T-Zell-Aktivierung-Experimenten.

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Immunologie und Infektion Ausgabe 144 einzelne Kette MHC menschliche T-Zell-Aktivierung T-Zell-Antigen-Anerkennung selbst-Peptid stabile Zelllinie veränderter antigenpräsentierende Zellen PBMC Isolation menschliche T-Zelle Degranulation Assay menschliche T-Zelle Cytokine Produktion
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