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Journal / Immunology and Infection / Användning av enda kedja MHC-teknik för att…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Användning av enda kedja MHC-teknik för att undersöka Co-agonism i mänskliga CD8 + T cellaktivering
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Användning av enda kedja MHC-teknik för att undersöka Co-agonism i mänskliga CD8 + T cellaktivering

Article DOI: 10.3791/59126-v • 12:09 min • February 28th, 2019
February 28th, 2019 •
1, 1, 1, 1,2, 3, 4, 3, 1,2,5, 6, 1, 1,2,5
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, 4Singapore Immunology Network, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, 6Department of Immunology, Wright-Fleming Institute, Imperial College London

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Det här protokollet beskriver användningen av enda kedja MHC klass I komplex att undersöka molekylära interaktioner i mänskliga CD8 + T cellaktivering: generation av bakåtkompilerade antigenpresenterande celler som uttrycker enda kedja konstruerar, kultur av mänskliga CD8 + T cell klon och T-cells aktivering experiment.

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Immunologi och infektion fråga 144 enda kedja MHC mänsklig T cellaktivering T cells antigen erkännande self peptid stabil cellinje konstruerade antigenpresenterande celler PBMC isolering mänsklig T cell degranulering assay mänsklig T cell cytokin produktion
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