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Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
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Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Article DOI: 10.3791/59139-v • 09:06 min • February 20th, 2019
February 20th, 2019 •
1, 1, 2,3, 1,4
1Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, 2Robert M. Berne Cardiovascular Research Center, University of Virginia, 3Department of Biochemistry and Molecular Genetics, University of Virginia, 4Division of Cardiology, University of Pittsburgh School of Medicine

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Summary
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We propose a standardized protocol to characterize the cellular composition of late-stage murine atherosclerotic lesions including systematic methods of animal dissection, tissue embedding, sectioning, staining, and analysis of brachiocephalic arteries from atheroprone smooth muscle cell lineage tracing mice.

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Quantitative Analysis Cellular Composition Atherosclerotic Lesions Smooth Muscle Cell Lineage-tracing Mice Experimental Pipelines Phenotypic Characterization Immunofluorescent Staining Qualitative Analysis Quantitative Analysis Phenotypes Brachiocephalic Artery Harvest Peritoneum Thorax Diaphragm Profusion Vessel Morphology Paraformaldehyde PBS
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