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Analyse quantitative de la Composition cellulaire dans les lésions athérosclérotiques avancées de Muscle lisse Cell Lineage-Tracing souris
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Analyse quantitative de la Composition cellulaire dans les lésions athérosclérotiques avancées de Muscle lisse Cell Lineage-Tracing souris

Article DOI: 10.3791/59139-v • 09:06 min • February 20th, 2019
February 20th, 2019 •
1, 1, 2,3, 1,4
1Heart, Lung, Blood and Vascular Medicine Institute, University of Pittsburgh, 2Robert M. Berne Cardiovascular Research Center, University of Virginia, 3Department of Biochemistry and Molecular Genetics, University of Virginia, 4Division of Cardiology, University of Pittsburgh School of Medicine

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Nous vous proposons un protocole normalisé afin de caractériser la composition cellulaire des stades des lésions athéroscléreuses murines y compris des méthodes systématiques de dissection animale, incorporation de tissus, coupes, coloration et analyse des artères brachiocéphalique atheroprone muscle lisse cell lineage traçage souris.

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Médecine question 144 athérosclérose lésion athéroscléreuse artère brachiocéphalique préparation du tissu et sectionnement immunofluorescence l’immunohistochimie microscopie confocale
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