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En alsidig metode til montering Arabidopsis blade til Intravital Time-lapse Imaging
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En alsidig metode til montering Arabidopsis blade til Intravital Time-lapse Imaging

Article DOI: 10.3791/59147-v • 07:52 min • February 11th, 2019
February 11th, 2019 •
1,2,3,4, 3,4, 2
1Japan Science and Technology Agency (JST), PRESTO, 2Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 3Faculty of Life and Environmental Sciences, University of Tsukuba, 4Microbiology Research Center for Sustainability, University of Tsukuba

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Vi rapporterer en simpel og alsidig metode til at udføre fluorescerende live-billeddannelse af Arabidopsis thaliana blade over en længere periode. Vi bruger en gensplejsede Arabidopsis plante at udtrykke et fluorescerende reporter gen under kontrol af en immunitet-relaterede promotor som et eksempel for at få spatiotemporelle forståelse af plante immunrespons.

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Biologi spørgsmålet 144 live-imaging time-lapse imaging blad Arabidopsis thaliana immunitet fluorescerende promotor-reporter
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