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Journal / Bioengineering / Zaaien en implantatie van een Biosynthetic…
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JoVE Journal Bioengineering
Zaaien en implantatie van een Biosynthetic weefsel-engineered tracheale Graft in een muismodel
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Zaaien en implantatie van een Biosynthetic weefsel-engineered tracheale Graft in een muismodel

Article DOI: 10.3791/59173-v • 09:57 min • April 1st, 2019
April 1st, 2019 •
*1,2, *1,3, 1,2, 4, 5, 3,6, 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital
* These authors contributed equally

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Graft stenose vormt een kritische obstakel in weefsel ontworpen airway vervanging. Om te onderzoeken cellulaire mechanismen ten grondslag liggen aan stenose, gebruiken we een lymfkliertest model van weefsel ontworpen tracheale vervanging met geplaatste beenmerg mononucleaire cellen (BM-MNC). Hier, detail we onze protocol, met inbegrip van de steiger productie, BM-MNC isolatie, graft zaaien en implantatie.

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Bioengineering kwestie 146 electrospinning kunststof steiger geneeskunde tracheale graft cel zaaien biomaterialen tracheale ziekten
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