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从 lck 中分离恶性和非恶性 b 细胞
 
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从 lck 中分离恶性和非恶性 b 细胞: egfp斑马鱼

Article DOI: 10.3791/59191-v 08:32 min February 22nd, 2019
February 22nd, 2019

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Summary

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转基因lck: egfp斑马鱼在 t 淋巴细胞中表达 gfp 的高度, 并已被用于研究 t 细胞发育和急性淋巴细胞白血病。这条线可以用来研究 b 细胞, b 细胞在较低的水平上表达lck 。该协议描述了从lck: egfp斑马鱼的恶性和非恶性 b 细胞的纯化。

Tags

医学 第144期 斑马鱼 d.rerio 白血病 急性淋巴细胞白血病 淋巴细胞 流式细胞术
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