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Journal / Biochemistry / Lo studio di RNA interattori della chinasi…
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Biochemistry

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JoVE Journal Biochemistry
Lo studio di RNA interattori della chinasi proteica RNA-attivato durante il ciclo cellulare dei mammiferi
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Lo studio di RNA interattori della chinasi proteica RNA-attivato durante il ciclo cellulare dei mammiferi

Article DOI: 10.3791/59215-v • 10:05 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1
1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

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Vi presentiamo approcci sperimentali per studi interattori di RNA double-stranded RNA binding della chinasi di proteina RNA-attivato (PKR) durante il ciclo cellulare dei mammiferi utilizzando cellule HeLa. Questo metodo utilizza la formaldeide a crosslink RNA-PKR complessi e immunoprecipitazione per arricchire associato a PKR RNAs. Questi RNA possono essere ulteriormente analizzati mediante sequenziamento ad alte prestazioni o qRT-PCR.

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Biochimica problema 145 Double-stranded RNA RNA mitocondriale RNA vincolante proteine RNA-RBP interazione proteina chinasi RNA-attivato formaldeide reticolazione ciclo cellulare qRT-PCR strand-specific
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