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Journal / Immunology and Infection / 重组呼吸道合胞病毒的生成、扩增和滴定…
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Immunology and Infection

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JoVE Journal Immunology and Infection
重组呼吸道合胞病毒的生成、扩增和滴定
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Journal (Immunology and Infection)
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
Journal (Immunology and Infection)
Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
 
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重组呼吸道合胞病毒的生成、扩增和滴定

Article DOI: 10.3791/59218-v • 11:48 min • April 4th, 2019
April 4th, 2019 •
1, 1, 1, 1, 1, 2, 1,3, 1,3
1UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin, 2UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, 3Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie

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我们描述了一种生成和扩增转基因呼吸道合胞病毒 (Rsv) 的方法和 Rsv 的优化斑块检测方法。我们通过创建两种重组病毒来说明这一协议, 它们分别允许对 RSV 复制进行定量, 并对 RSV 包涵体和包涵体相关颗粒动力学进行实时分析。

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免疫学与感染 第146期 反向遗传学 呼吸道合胞病毒 感染 重组病毒 放大 滴定 标签 荧光 定量 视频显微镜
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