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JoVE Journal Bioengineering
3D-analyse av multi-Cellular Svar å Chemoattractant graderinger
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3D-analyse av multi-Cellular Svar å Chemoattractant graderinger

Article DOI: 10.3791/59226-v • 05:57 min • May 24th, 2019
May 24th, 2019 •
1, 2, 1, 2,3, 1
1Department of Biomedical Engineering and Yale Systems Biology Institute, Yale University, 2Department of Biomedical Engineering, Johns Hopkins University, 3Center for Cell Dynamics and Department of Cell Biology, Johns Hopkins University

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Vi beskriver en metode for å konstruere enheter for 3D-kultur og eksperimentering med celler og flercellede organoids. Denne enheten tillater analyse av cellulære reaksjoner på oppløselige signaler i 3D-microenvironments med definerte chemoattractant graderinger. Organoids er bedre enn enkeltceller ved påvisning av svake støyende innganger.

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Bioteknologi gradient sensing organer-on-a-chip chemotaxis Stereolithography ekstracellulære matrise utvikling organoids
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