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Surexprimant ARN Long non codantes en utilisant activation génique CRISPR

Article DOI: 10.3791/59233-v • 13:04 min • March 1st, 2019
March 1st, 2019 •
1, 1, 2, 1, 3, 3, 1, 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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Techniques traditionnelles axées sur l’ARNC surexpression ont une portée limitée pour la surexpression de l’ARN long non codantes en raison de leurs multiples formes d’épissure avec fonctionnalité potentielle. Cette étude rapporte un protocole utilisant la technologie CRISPR pour surexprimer multiples variants d’épissage d’un ARN long non codant.

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Génétique numéro 145 lncRNA CRISPR IFNG-AS1 surexpression PCR des gènes en temps réel
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