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Journal / Genetics / Overexpressing lang Noncoding RNAs med aktivering …
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JoVE Journal Genetics
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Overexpressing lang Noncoding RNAs med aktivering av Gene CRISPR

Article DOI: 10.3791/59233-v • 13:04 min • March 1st, 2019
March 1st, 2019 •
1, 1, 2, 1, 3, 3, 1, 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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Tradisjonelle cDNA-baserte overuttrykte teknikker har et begrenset bruksområde for overuttrykte lang noncoding RNAs på grunn av skjemaene for flere skjøte med mulig funksjonalitet. Denne anmeldelsen rapporterer en protokoll bruker CRISPR teknologi for å overexpress flere skjøte-varianter av en lang noncoding.

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Genetikk problemet 145 lncRNA CRISPR IFNG-AS1 overuttrykte lentiviral sanntids PCR
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