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Monster voorbereiding methode van scannen en transmissie elektronenmicroscoop voor de aanhangsels van woodboring Kever
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Monster voorbereiding methode van scannen en transmissie elektronenmicroscoop voor de aanhangsels van woodboring Kever

Article DOI: 10.3791/59251-v • 10:09 min • February 3rd, 2020
February 3rd, 2020 •
1,2, 3, 1, 4, 1
1The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, 2School of Forestry, Inner Mongolia Agricultural University, 3Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 4School of Forestry, Fujian Agriculture and Forestry University

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Om ultrastructuur van insectensensilla te observeren, werd in de studie het protocol voor de voorbereiding van de insectenensilla, scanning en transmissieelektronenmicroscopie (SEM en TEM) in de studie gepresenteerd. Tween 20 werd toegevoegd aan de fixatie om monstervervorming in SEM te voorkomen. Fluorescentiemicroscopie was nuttig voor het verbeteren van de snijnauwkeurigheid in TEM.

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Milieuwetenschappen Kwestie 156 insect reuk gustatory sensilla ultrastructuur het aftasten elektronenmicroscoop transmissieelektronenmicroscoop fluorescentiemicroscoop chemische ecologie
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