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Journal / Neuroscience / In vivo tredimensionel to-foton mikroskopi til…
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Neuroscience

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JoVE Journal Neuroscience
In vivo tredimensionel to-foton mikroskopi til undersøgelse udført vaskulære reaktioner ved lokal ATP udslyngning ved hjælp af en glas mikro-pipette
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In vivo tredimensionel to-foton mikroskopi til undersøgelse udført vaskulære reaktioner ved lokal ATP udslyngning ved hjælp af en glas mikro-pipette

Article DOI: 10.3791/59286-v • 10:54 min • June 7th, 2019
June 7th, 2019 •
1, 1, 1, 1, 1, 1, 1,2
1Department of Neuroscience, Faculty of Medicine and Health Science, University of Copenhagen, 2Department of Clinical Neurophysiology, Rigshospitalet

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Vi præsenterer en optimeret lokal udslyngning procedure ved hjælp af en glas mikro-pipette og en hurtig to-photon hyperstack Imaging metode, som tillader præcis måling af kapillar diameterændringer og undersøgelse af dens regulering i tre dimensioner.

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Neurovidenskab in vivo to-photon mikroskopi cerebral blodgennemstrømning (CBF) astrocytter tre dimensioner (3D) neurovaskulær kobling calcium Imaging viral vektor injektion
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