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Fluorescentie herstel na Photobleaching van gele fluorescerende eiwit Tagged p62 in Aggresome-achtige geïnduceerde structuren

Article DOI: 10.3791/59288-v • 12:58 min • March 26th, 2019
March 26th, 2019 •
1, 2, 2
1Core Imaging Facility and Department of Microbiology and Immunology, Loyola University Chicago, 2Department of Molecular Pharmacology and Therapeutics, Loyola University Chicago

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We beschrijven een uitgebreide en praktische protocol voor fluorescentie herstel na photobleaching experimenten met cellen levende. Hoewel het protocol werd gebruikt voor het meten van de mobiliteit van gele fluorescerende eiwit-gelabeld p62 in aggresome-achtige geïnduceerde structuren, kan het worden toegepast op een verscheidenheid van microscopie systemen en fluorescente proteïnen.

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Biologie kwestie 145 fluorescentie herstel na photobleaching FRAP gele fluorescerende eiwit levende cel imaging eiwit mobiliteit lymfkliertest macrofagen p62 sequestosome-1 aggresome
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