Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / A New Best Practice for Validating Tail Vein…
JoVE Journal
Medicine

This content is Open Access.

JoVE Journal Medicine
A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
Intra-Operative Neural Monitoring of Thyroid Surgery in a Porcine Model
Journal (Medicine)
Intra-Operative Neural Monitoring of Thyroid Surgery in a Porcine Model
Murine Model of Wound Healing
Journal (Medicine)
Murine Model of Wound Healing
 

A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

Article DOI: 10.3791/59295-v • 04:19 min • April 19th, 2019
April 19th, 2019 •
*1,2,3, *1,2,3, 1,2,3, 3,4, 3,4, 1,2,3
1Bayer School of Natural and Environmental Sciences, Duquesne University, 2Department of Biological Sciences, Duquesne University, 3Chronic Pain Research Consortium, Duquesne University, 4Graduate School of Pharmaceutical Sciences, Duquesne University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Here we present a method to validate tail vein injections in rats by utilizing near-infrared fluorescence imaging data from dyes incorporated into agents or biological probes. The tail is imaged before and after the injection, the fluorescent signal is quantified, and an assessment of the injection quality is made.

Tags

Tail Vein Injections Near-infrared-labeled Agents Injection Accuracy Quality Control Animal Studies Fluorescence Imaging Blood Circulation Visual Demonstration Injected Agent Clearance Muzamil Saleem Brooke Deal PhD Student Anesthetized Animal Fluorescence Imager Injection Site Exposure Tail Vasculature Dilation Alcohol Pads 25 To 27-gauge Needle
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter