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JoVE Journal
Developmental Biology

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Generation af 3D hud Organoid fra ledningen blod-afledte induceret pluripotente stamceller
 
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Generation af 3D hud Organoid fra ledningen blod-afledte induceret pluripotente stamceller

Article DOI: 10.3791/59297-v 09:54 min April 18th, 2019
April 18th, 2019

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Summary

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Click here for the English version.

Vi foreslår en protokol, der viser hvordan du differentiere inducerede pluripotente stamceller-afledt keratinocytter og fibroblaster og generere en 3D hud organoid, ved hjælp af disse keratinocytter og fibroblaster. Denne protokol indeholder en yderligere skridt til at generere en humaniseret mus model. Teknikken præsenteret her vil forbedre dermatologiske forskning.

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Udviklingsmæssige biologi spørgsmålet 146 Induced pluripotente stamceller ledningen blod mononukleære celler fibroblast keratinocytter 3D hud organoid skin graft humaniseret mus model
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