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JoVE Journal Neuroscience
Conversión de células madre pluripotentes inducidas por humanos (IPSC) en neuronas motoras craneales y espinales funcionales usando vectores PiggyBac
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Conversión de células madre pluripotentes inducidas por humanos (IPSC) en neuronas motoras craneales y espinales funcionales usando vectores PiggyBac

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Este protocolo permite la conversión rápida y eficiente de células madre pluripotentes inducidas en neuronas motoras con una identidad espinal o craneal, mediante la expresión ectópica de factores de transcripción de vectores piggyBac inducibles.

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Neurociencia problema 147 células madre pluripotentes inducidas neuronas motoras piggyBac diferenciación factores de transcripción Phox2a Isl1 Ngn2 Lhx3
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