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Journal / Neuroscience / Conversion de cellules souches pluripotentes…
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Neuroscience

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JoVE Journal Neuroscience
Conversion de cellules souches pluripotentes induites par l’homme (iPSCs) en neurones moteurs spinaux et crânien fonctionnels utilisant des vecteurs PiggyBac
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
Journal (Neuroscience)
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
Journal (Neuroscience)
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
 
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Conversion de cellules souches pluripotentes induites par l’homme (iPSCs) en neurones moteurs spinaux et crânien fonctionnels utilisant des vecteurs PiggyBac

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Ce protocole permet une conversion rapide et efficace des cellules souches pluripotentes induites en neurones moteurs avec une identité spinale ou crânienne, par l’expression ectopique des facteurs de transcription des vecteurs inductibles de piggyBac.

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Neurosciences numéro 147 cellules souches pluripotentes induites neurones moteurs piggyBac différenciation facteurs de transcription Phox2a Isl1 Ngn2 Lhx3
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