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JoVE Journal Neuroscience
Conversie van menselijke geïnduceerde pluripotente stamcellen (iPSCs) in functionele spinale en craniale motor neuronen met behulp van PiggyBac vectoren
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Conversie van menselijke geïnduceerde pluripotente stamcellen (iPSCs) in functionele spinale en craniale motor neuronen met behulp van PiggyBac vectoren

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Dit protocol maakt een snelle en efficiënte omzetting van geïnduceerde pluripotente stamcellen in motorische neuronen met een wervelkolom of schedel identiteit, door buitenbaarmoederlijke expressie van transcriptiefactoren van afleidbaar piggyBac vectoren.

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Neurowetenschappen geïnduceerde pluripotente stamcellen motor neuronen piggyBac differentiatie transcriptiefactoren Phox2a Isl1 Ngn2 Lhx3
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