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Utilisation de la coloration en immunofluorescence pour observer directement les cellules endothéliales vasculaires

Article DOI: 10.3791/59325-v • 06:09 min • August 20th, 2019
August 20th, 2019 •
*1,2, *1,2, 1,2, 1,2, 1, 1, 1, 1, 1, 1
1Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 2Institute of Cardiovascular Diseases, Shanghai Jiao Tong University
* These authors contributed equally

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Ici, nous présentons un protocole pour la coloration d'immunofluorescence pour observer les cellules endothéliales de l'aorte de souris directement. Cette technique est utile lors de l'étude du phénotype cellulaire et moléculaire des cellules endothéliales dans différents schémas de flux et dans le développement de l'athérosclérose.

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Biologie Numéro 150 En visage immunofluorescence aorte endothéliale morphologie in situ athérosclérose
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