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JoVE Journal Cancer Research
En Ortotopisk endometriecancer model med retroperitoneal lymfadenopati fremstillet af in vivo opformeret og kulturperler VX2-celler
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En Ortotopisk endometriecancer model med retroperitoneal lymfadenopati fremstillet af in vivo opformeret og kulturperler VX2-celler

Article DOI: 10.3791/59340-v • 09:48 min • September 12th, 2019
September 12th, 2019 •
1,2, 3, 4, 5, 6, 5,6, 5,7,8, 7,9,11, 10, 5,11
1Institute of Medical Science, University of Toronto, 2Department of Obstetrics and Gynecology, University of Toronto, 3Guided Therapeutics Laboratory, TECHNA Institute, University Health Network, 4Department of Pathology, Princess Margaret Cancer Center, University Health Network, 5Department of Medical Biophysics, University of Toronto, 6Princess Margaret Cancer Center, University Health Network, 7Techna Institute, University Health Network, 8Department of Surgery, University of Toronto, 9Department of Otolaryngology, Head and Neck Surgery, University of Toronto, 10Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center, University Health Network, 11Princess Margaret Cancer Center, University Health Network

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Denne protokol præsenterer en standardiseret metode til at dyrke VX2-celler i kulturen og til at skabe en ortotopisk VX2-model af endometriecancer med retroperitoneal lymfeknude metastaser hos kaniner. Ortotopiske endometriale Cancer modeller er vigtige for den prækliniske undersøgelse af nye billedbehandlings metoder til diagnosticering af lymfeknudemetastaser.

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Kræftforskning endometriecancer Orthotopic Cancer model VX2-cellelinje lymfadenopati kanin model kirurgisk model cellekultur
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