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Ein orthotopisches Endometriumkrebs-Modell mit retroperitonealer Lymphadenopathie made From In Vivo Propagated and Cultured VX2 Cells

Article DOI: 10.3791/59340-v • 09:48 min • September 12th, 2019
September 12th, 2019 •
1,2, 3, 4, 5, 6, 5,6, 5,7,8, 7,9,11, 10, 5,11
1Institute of Medical Science, University of Toronto, 2Department of Obstetrics and Gynecology, University of Toronto, 3Guided Therapeutics Laboratory, TECHNA Institute, University Health Network, 4Department of Pathology, Princess Margaret Cancer Center, University Health Network, 5Department of Medical Biophysics, University of Toronto, 6Princess Margaret Cancer Center, University Health Network, 7Techna Institute, University Health Network, 8Department of Surgery, University of Toronto, 9Department of Otolaryngology, Head and Neck Surgery, University of Toronto, 10Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center, University Health Network, 11Princess Margaret Cancer Center, University Health Network

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Dieses Protokoll stellt eine standardisierte Methode zum Wachstum von VX2-Zellen in Kultur dar und zur Erstellung eines orthotopischen VX2-Modells von Endometriumkrebs mit retroperitonealen Lymphknotenmetastasen bei Kaninchen. Orthotopische Endometriumkrebsmodelle sind wichtig für die präklinische Untersuchung neuartiger bildgebender Modalitäten für die Diagnose von Lymphknotenmetastasen.

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Krebsforschung Ausgabe 151 Endometriumkrebs Orthotopisches Krebsmodell VX2-Zelllinie Lymphadenopathie Kaninchenmodell Chirurgisches Modell Zellkultur
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