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Journal / Bioengineering / CRISPR/Cas12a multiplex genoom bewerking van…
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Bioengineering

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JoVE Journal Bioengineering
CRISPR/Cas12a multiplex genoom bewerking van Saccharomyces cerevisiae en de creatie van gist pixel art
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CRISPR/Cas12a multiplex genoom bewerking van Saccharomyces cerevisiae en de creatie van gist pixel art

Article DOI: 10.3791/59350-v • 10:18 min • May 28th, 2019
May 28th, 2019 •
1,2, 1, 3,4, 1, 1
1DSM Biotechnology Center, 2Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, 3BrisSynBio, University of Bristol, Life Sciences Building, 4School of Biological Sciences, University of Bristol, Life Sciences Building

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Het CRISPR/Cas12a systeem in combinatie met een enkele crRNA array maakt efficiënte multiplex bewerking van de S. cerevisiae genoom op meerdere loci tegelijk mogelijk. Dit wordt aangetoond door het construeren van Carotenoïde producerende giststammen die vervolgens worden gebruikt om gist pixel kunst te maken.

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Bioengineering CRISPR/Cas12a CRISPR/Cpf1 CRISPR/Cas9 multiplex genoom Editing Saccharomyces cerevisiae gist pixel art
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