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Journal / Developmental Biology / Isolation and Staining of Mouse Skin…
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JoVE Journal Developmental Biology
Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

Article DOI: 10.3791/59353-v • 12:34 min • May 9th, 2019
May 9th, 2019 •
1, 1
1Department of Dermatology and Charles C. Gates Center for Regenerative Medicine, University of Colorado Anschutz Medical Campus

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This protocol describes how to isolate skin keratinocytes from mouse models, to stain with metal-tagged antibodies, and to analyze stained cells by mass cytometry in order to profile the expression pattern of proteins of interest in the different cell cycle phases.

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Isolation Staining Mouse Skin Keratinocytes Cell Cycle Protein Expression Mass Cytometry Cell Proliferation Markers Phases Cancer Research Disease Cell Types Model Organisms Cell Preparation Metal-tagged Antibody Panel IdU Stock Solution
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