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Journal / Genetics / Isolering af Papillar og Rekulære fibroblaster…
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Genetics

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JoVE Journal Genetics
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Isolering af Papillar og Rekulære fibroblaster fra menneskehud ved fluorescens-aktiveret celle sortering

Article DOI: 10.3791/59372-v • 09:14 min • May 7th, 2019
May 7th, 2019 •
*1, *1, 1
1Skin, Endothelium Research Division, Department of Dermatology, Medical University of Vienna
* These authors contributed equally

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Dette manuskript beskriver en FACS-baseret protokol til isolering af papillar og rekulære fibroblaster fra menneskehud. Det omgår in vitro-kultur, som var uundgåelig med den almindeligt anvendte isolation protokol via eksplantat kulturer. De udstammede fibroblast undersæt er funktionelt adskilte og udviser differentialgenekspression og lokalisering inden for dermis.

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Genetik FACS celleoverflade markører fibroblast heterogenitet fibroblast lineages menneskehud papillar og rekulære fibroblaster
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