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Journal / Genetics / HOX Loci fokuserad CRISPR/sgRNA bibliotek…
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JoVE Journal Genetics
HOX Loci fokuserad CRISPR/sgRNA bibliotek Screening identifiera kritiska SAP gränser
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HOX Loci fokuserad CRISPR/sgRNA bibliotek Screening identifiera kritiska SAP gränser

Article DOI: 10.3791/59382-v • 10:10 min • March 31st, 2019
March 31st, 2019 •
1, 2, 2, 1, 1, 3, 1
1Department of Pediatrics, Pennsylvania State University College of Medicine, 2Department of Physiological Sciences, University of Florida, 3Department of Anatomy and Cell Biology, University of Florida

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CRISPR/sgRNA bibliotek har tillämpats på förhör protein-kodande gener. Genomförbarheten av ett sgRNA-bibliotek för att avslöja funktionen av en SAP gräns i genreglering är dock fortfarande outforskat. Här beskriver vi ett HOX loci specifika sgRNA bibliotek för att belysa funktionen av SAP gränser i HOX loci.

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Genetik fråga 145 CRISPR/Cas9 sgRNA bibliotek screening SAP gräns HOX loci one-step RT-qPCR ditsatta mutation upptäckt akut myeloisk leukemi
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