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HOX Loci gericht CRISPR/sgRNA bibliotheek Screening identificeren kritische CTCF grenzen

Article DOI: 10.3791/59382-v • 10:10 min • March 31st, 2019
March 31st, 2019 •
1, 2, 2, 1, 1, 3, 1
1Department of Pediatrics, Pennsylvania State University College of Medicine, 2Department of Physiological Sciences, University of Florida, 3Department of Anatomy and Cell Biology, University of Florida

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Een CRISPR/sgRNA-bibliotheek is toegepast op het ondervragen van eiwit-codeert genen. De haalbaarheid van een bibliotheek van de sgRNA aan het licht brengen van de functie van een scheidingswand CTCF in genregulatie blijft echter onontgonnen. Hier beschrijven we een HOX loci specifieke sgRNA bibliotheek te verhelderen van de functie van CTCF grenzen in HOX loci.

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Genetica kwestie 145 CRISPR/Cas9 sgRNA bibliotheek screening CTCF grens HOX loci one-step RT-qPCR Indel mutatie detectie Acute myeloïde leukemie
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