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HOX Loci Focused CRISPR/sgRNA biblioteca rastreio identificando crítico CTCF limites
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HOX Loci Focused CRISPR/sgRNA biblioteca rastreio identificando crítico CTCF limites

Article DOI: 10.3791/59382-v • 10:10 min • March 31st, 2019
March 31st, 2019 •
1, 2, 2, 1, 1, 3, 1
1Department of Pediatrics, Pennsylvania State University College of Medicine, 2Department of Physiological Sciences, University of Florida, 3Department of Anatomy and Cell Biology, University of Florida

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Uma biblioteca CRISPR/sgRNA aplicou-se para interrogar os genes codificantes de proteínas. No entanto, a viabilidade de uma biblioteca de sgRNA para descobrir a função de um limite CTCF na regulação gênica permanece inexplorada. Aqui, descrevemos uma biblioteca de sgRNA específico de loci HOX para elucidar a função dos limites CTCF HOX loci.

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Genética edição 145 CRISPR/Cas9 triagem de biblioteca sgRNA limite CTCF HOX loci uma etapa RT-qPCR deteção de mutação Indel leucemia mieloide aguda
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