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Journal / Biochemistry / In Vitro Ubiquitination und Deubiquitination…
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Biochemistry

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JoVE Journal Biochemistry
In Vitro Ubiquitination und Deubiquitination Assays von Nucleleosomalhistones
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Journal (Biochemistry)
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Cryo-EM and Single-Particle Analysis with Scipion
Journal (Biochemistry)
Cryo-EM and Single-Particle Analysis with Scipion
 
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In Vitro Ubiquitination und Deubiquitination Assays von Nucleleosomalhistones

Article DOI: 10.3791/59385-v • 11:36 min • July 25th, 2019
July 25th, 2019 •
*1, *1, 1, 1, 1, 1, 1, 1,2, 1,2
1Maisonneuve-Rosemont Hospital Research Center and Department of Medicine, University of Montréal, 2Lunenfeld-Tanenbaum Research Institute, Sinai Health System
* These authors contributed equally

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Ubiquitination ist eine post-translationale Modifikation, die eine wichtige Rolle in zellulären Prozessen spielt und durch Deubiquitination eng koordiniert wird. Defekte in beiden Reaktionen liegen den menschlichen Pathologien zugrunde. Wir bieten Protokolle zur Durchführung von Ubiquitination und Deubiquitination Reaktion in vitro mit gereinigten Komponenten.

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Biochemie Ausgabe 149 Ubiquitination Deubiquitination E3 ubiquitin ligase PRC1 deubiquitinase PR-DUB Chromatin BAP1 ASXL BMI1 RING1B H2A
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