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Immunology and Infection
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JoVE Journal Immunology and Infection
Design von Cecal Ligation und Punktion und Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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Design von Cecal Ligation und Punktion und Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

Article DOI: 10.3791/59386-v • 07:30 min • June 15th, 2019
June 15th, 2019 •
*1,2, *1,2, *2,3, 1, 3, 2
1West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 2Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University
* These authors contributed equally

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Dieses Protokoll beschreibt Techniken zur Messung von infektiösen Ergebnissen, die sekundären Krankenhausinfektionen im immunsuppressiven Zustand zugrunde liegen, indem sie zunächst Cecal-Ligations-/Punktionsmäuse einrichten und sie mit intranasalen Infektionen herausfordern, um ein klinisch relevantes Modell der Immunsuppressionssepsis zu erstellen.

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Immunologie und Infektion Ausgabe 148 Cecal Ligation und Punktion intranasale Infektion Sepsis-induzierte Immunsuppression Immunsuppression Sepsis Sepsis Staphylococcus aureus nosokomiale Lungenentzündungen Mäuse Doppelmodell Doppelschlagmodell
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