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Biochemistry

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JoVE Journal Biochemistry
Misurazione diretta del coinvolgimento target KDM1A utilizzando Immunoassays basate su Chemoprobe
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Misurazione diretta del coinvolgimento target KDM1A utilizzando Immunoassays basate su Chemoprobe

Article DOI: 10.3791/59390-v • 11:17 min • June 13th, 2019
June 13th, 2019 •
1, 1, 1
1Oryzon Genomics

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Qui, presentiamo un protocollo per misurare kDM1A target engagement in una cellula umana o animale, tessuto o campioni di sangue trattati con inibitori KDM1A. Il protocollo utilizza l'etichettatura chemioprobe dell'enzima KDM1A gratuito e la quantificazione diretta dell'occupazione target utilizzando immunoasisti basati sulla chemiospa e può essere utilizzato in studi preclinici e clinici.

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Biochimica Numero 148 Impegno obiettivo Modifiche Histone Istone Demethylases KDM1A Chemoprobe ELISA
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