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Journal / Biochemistry / 血管紧张蛋白II图解的硝酸分析与鉴定
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Biochemistry

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JoVE Journal Biochemistry
血管紧张蛋白II图解的硝酸分析与鉴定
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血管紧张蛋白II图解的硝酸分析与鉴定

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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由于三硝基酪氨酸修饰的丰度较低,酪氨酸硝酸蛋白的蛋白组谱分析一直是一项具有挑战性的技术。在这里,我们描述了一种利用血管紧张蛋白II作为模型来增强和分析硝基肽扩脂的新方法。此方法可以扩展到其他体外或体内系统。

Tags

生物化学 问题 148 硝基肽 血管紧张症 II 硝基酪氨酸 二甲基标签 二甲酸钠 生物锡标签 浓缩
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