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Journal / Medicine / Visualisierung von endogenen Mitophagie-Komplexen …
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Visualisierung von endogenen Mitophagie-Komplexen in Situ in menschlichen Pankreas-Betazellen unter Verwendung von Proximity Ligation Assay
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Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood
Journal (Medicine)
Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood
 
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Visualisierung von endogenen Mitophagie-Komplexen in Situ in menschlichen Pankreas-Betazellen unter Verwendung von Proximity Ligation Assay

Article DOI: 10.3791/59398-v • 08:40 min • May 2nd, 2019
May 2nd, 2019 •
1, 1,2
1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, 2VA Ann Arbor Healthcare System

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Dieses Protokoll skizziert eine Methode zur quantitativen Analyse der Mitophagie-Protein-Komplexbildung speziell in Beta-Zellen aus primären menschlichen Ischenproben. Diese Technik ermöglicht somit die Analyse der Mitophagie aus begrenztem biologischem Material, die in wertvollen menschlichen Betazellproben der Bauchspeicheldrüse entscheidend sind.

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Medizin Ausgabe 147 Bauchspeicheldrüsen-Betazellen Mitophagie Proximity Ligation Assay menschliche Inselchen Protein-Interaktion Mitochondrien
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