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Orthotopic Rat Kidney Transplantation
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Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Article DOI: 10.3791/59403-v • 09:15 min • May 7th, 2019
May 7th, 2019 •
1, 1, 1, 1, 1, 1, 1
1Department of Surgery, Johns Hopkins University School of Medicine

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Summary
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The purpose of this manuscript and protocol is to explain and demonstrate in detail the surgical procedure of orthotopic kidney transplantation in rats. This method is simplified to achieve the correct perfusion of the donor kidney and shorten the reperfusion time by using the venous and ureteral cuff anastomosis technique.

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Orthotopic Rat Kidney Transplantation Surgical Approach Small Animal Transplantation Critical Steps Perfusion Portal Vein Cuff System Anastomose Renal Veins Ureters Anesthetize Supine Position Immobilize Limbs Surgical Field Incision Peritoneal Cavity Intestine Moist Sterile Gauze Aorta Vena Cava
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