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Journal / Biochemistry / Ionbytning kromatografi (IEX) koblet til…
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Biochemistry

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JoVE Journal Biochemistry
Ionbytning kromatografi (IEX) koblet til multi-vinkel lysspredning (MALS) for Protein adskillelse og karakterisering
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Ionbytning kromatografi (IEX) koblet til multi-vinkel lysspredning (MALS) for Protein adskillelse og karakterisering

Article DOI: 10.3791/59408-v • 10:41 min • April 5th, 2019
April 5th, 2019 •
1, 2, 3, 1
1Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, 2Wyatt Technology Corporation, 3Danyel Biotech Ltd

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Denne protokol beskriver brug af høj-specificitet ionbytning kromatografi med multi-vinkel lysspredning til en nøjagtig molar masse bestemmelse af proteiner, proteinkomplekser og peptider i en heterogen prøve. Denne metode er værdifuld for kvalitetsvurdering, såvel som for karakterisering af indfødte oligomerer, charge varianter og blandet protein prøver.

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Biokemi spørgsmålet 146 ionbytning (IEX) multi-vinkel lysspredning (MALS) kromatografi protein adskillelse molekylvægt bovint serumalbumin (BSA) oligomerer kvalitetskontrol
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