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Developmental Biology

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JoVE Journal Developmental Biology
Analyse af hjerte kammer udvikling under musen embryogenese bruger hele bjerget epifluorescensmikroskop
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Analyse af hjerte kammer udvikling under musen embryogenese bruger hele bjerget epifluorescensmikroskop

Article DOI: 10.3791/59413-v • 06:27 min • April 17th, 2019
April 17th, 2019 •
1,2,3, 1,2,3
1Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology, Vanderbilt University

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Vi præsenterer protokollerne for at undersøge mus hjerte udvikling ved hjælp af hele mount epifluorescerende mikroskopi på musen embryoner dissekeret fra ventrikulær specifikke MLC-2v-tdTomato reporter knock-i mus. Denne metode gør det muligt for os at direkte visualisere hvert trin af den ventrikulære dannelse under musen hjerte udvikling uden arbejdskrævende histokemiske metoder.

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Udviklingsmæssige biologi spørgsmålet 146 hjerte Embryo ventrikel kammer hele mount og epifluorescensmikroskop
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