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JoVE Journal Developmental Biology
Analyse av Cardiac kammer utvikling under musen Embryogenesis bruke hele Mount Epifluorescence
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Dissection of Drosophila melanogaster Third Instar Larval Brain for Live-Cell Imaging (Video) | JoVE
Journal (Developmental Biology)
Dissection of Drosophila melanogaster Third Instar Larval Brain for Live-Cell Imaging (Video) | JoVE
 
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Analyse av Cardiac kammer utvikling under musen Embryogenesis bruke hele Mount Epifluorescence

Article DOI: 10.3791/59413-v • 06:27 min • April 17th, 2019
April 17th, 2019 •
1,2,3, 1,2,3
1Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology, Vanderbilt University

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Vi presenterer protokoller for å undersøke musen heart utvikling med hele mount epifluorescent mikroskopi på mouse embryoer dissekert fra ventrikkel bestemt MLC-2v-tdTomato reporter banke i mus. Denne metoden tillater oss å visualisere direkte hvert trinn av ventrikkel formasjonen under musen heart utvikling uten arbeidskrevende histochemical metoder.

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Utviklingspsykologi biologi problemet 146 hjertet Embryo ventrikkel kammer hele mount og Epifluorescence
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