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Journal / Genetics / Brug af enkelt molekyle fluorescerende i Situ…
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JoVE Journal Genetics
Brug af enkelt molekyle fluorescerende i Situ hybridisering (SM-fisk) til tal og Oversæt mRNAs i Murine oocyter
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Brug af enkelt molekyle fluorescerende i Situ hybridisering (SM-fisk) til tal og Oversæt mRNAs i Murine oocyter

Article DOI: 10.3791/59414-v • 08:18 min • April 24th, 2019
April 24th, 2019 •
1, 1
1Department of Animal Science, University of Nebraska-Lincoln

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Hvis du vil reproducerbar tælle antallet af mRNAs i individuelle oocytter, enkelt molekyle RNA fluorescens in situ var hybridisering (RNA-fisk) optimeret til ikke-tilhænger celler. Oocytter blev indsamlet, hybridiseret med udskrift specifikke sonder og kvantificeres ved hjælp af et billede kvantificering software.

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Genetik spørgsmålet 146 oocyt mRNA kvantificering mRNA lokalisering ikke-tilhænger celler fluorescens in situ hybridisering forgrenede DNA
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